PDA

View Full Version : Distribution of Four HIV Type 1-Resistance Polymorphisms in the Bahraini Population



Sisyphus
2010-12-23, 14:01
Abstract

Allelic differences of chemokine (C-C motif ) receptor 5 (CCR5) and CCR2, as well as the ligand for the chemokinereceptor CXCR4, stromal-derived factor (SDF-1), are known to suppress HIV-1 transmission and to be involved indelay in HIV-1 disease progression. The aim of our study was to investigate the frequencies of four mutations thatconfer resistance to HIV-1: CCR5-D32, CCR5-m303, CCR2-64I, and SDF1-30A among Bahrainis. We have studied the DNA polymorphisms in 304 unrelated healthy Bahraini individuals without any known history of HIV-1 infection or AIDS symptoms. The CCR5-D32 mutation was detected by PCR analysis, while the CCR5-m303, CCR2-64I, and SDF1-30A mutations were detected by PCR-restriction fragment length polymorphism (PCR-RFLP) tests. Allele frequencies and the fit to the Hardy–Weinberg equilibrium were evaluated using the Arlequin populationgenetics application. The frequencies of the CCR5-D32, CCR2-64I, and SDF1-30A alleles were 2.8%, 8.9%, and 26.5%, respectively. No mutant alleles were detected for the CCR5-m303 mutation in 304 individuals. We esti-mated the risk of AIDS onset (relative hazard), computed from the three-locus genotype data. This is the firstreport of these four mutations conferring resistance to HIV-1 in the Bahraini population. The presence of the CCR5-D32 allele among Bahrainis may be attributed to the admixture with people of European descent. The CCR2-64I allele and especially the SDF1-30A allele are predominant in the Bahraini population and may be associated withresistance to fast HIV-1 infection in Bahrainis, and thus their genotyping can be used for prognosis in HIV-infected individuals.

Full Article (http://docs.google.com/viewer?a=v&q=cache:9Y_ZOzCsNTgJ:batzerlab.lsu.edu/publications/Salem_et_al_2009_AIDS_Res_Hum_Retroviruses.pdf+CCR 5-Delta+32+ethnic&hl=en&gl=us&pid=bl&srcid=ADGEESh_7R7oW2M54Vr7WRQ9KFFmHrICnWF2ByphwA7s baX9JJYy6Wr-F-xhgz6Jq3RTspC5BxKvP0AqcBYU2AdBx1GrtG10nQTLTsjEl5Lf x4HzeJ2EbiiIq6xQTlzZtf7hd7VJvboX&sig=AHIEtbSjeMQpDiWZ-wt2LowI9IcM6jvy9Q)